Journal: Translational Oncology
Article Title: Irinotecan Upregulates Fibroblast Growth Factor Receptor 3 Expression in Colorectal Cancer Cells, Which Mitigates Irinotecan-Induced Apoptosis 1 2
doi: 10.1016/j.tranon.2017.02.004
Figure Lengend Snippet: IRI-induced caspase activity. Cells were exposed to synergistic concentrations of IRI, PD173074, or both compounds for 72 hours before being lysed for determination of caspase 3 activity. Activity was calculated relative to the untreated control, and bars represent the mean ± SD of three independent experiments. Differences were analyzed by Student's t test with Welch correction. (A) SW480 control, (B) SW480 FGFR3-IIIc, (C) Caco-2, and (D) SW620 control.
Article Snippet: Shortly, protein was isolated from cells by homogenization in lysate buffer, and enzyme activity was measured by incubation with the specific fluorescent caspase substrate (sc-311,274, Santa Cruz Biotech) for 90 minutes.
Techniques: Activity Assay, Control